Analytical Data
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基因名
Cyclin E
- Application
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别名
CCNE; CCNE1; Cyclin E1; G1/S-specific cyclin-E1
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种属
Human
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表达系统
Baculovirus
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P24864
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表达区间
M1-A410
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氨基酸序列
MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
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蛋白长度
Full Length
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分子量
47.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclin E is a key regulatory protein that plays a pivotal role in the progression of the cell cycle, particularly in the transition from the G1 phase to the S phase. It functions by activating cyclin-dependent kinases (CDKs), specifically CDK2, which subsequently phosphorylates target proteins essential for DNA replication. Dysregulation of Cyclin E expression has been implicated in various cancers, as overexpression can lead to uncontrolled cell proliferation and genomic instability. Research on recombinant Cyclin E protein seeks to elucidate its structural and functional characteristics, enabling a better understanding of its role in normal cellular processes and its contributions to tumorigenesis. The availability of purified recombinant Cyclin E allows for detailed biochemical assays, structural studies, and potential therapeutic applications, such as the development of CDK inhibitors. Understanding the molecular mechanisms governing Cyclin E activity may provide insights into new cancer treatment strategies and the potential for targeted therapies aimed at restoring normal cell cycle regulation. The study of Cyclin E as a therapeutic target is particularly relevant in the context of tumor types associated with its aberrant expression, making it a significant focus in cancer research.
