Analytical Data
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Gene name
ECD
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简介
ECD proteins are multifunctional regulators of cellular dynamics that cooperate with TXNIP to stabilize and regulate p53/TP53 by inhibiting MDM2-mediated degradation. In addition to p53 stabilization, ECD is involved in complex transcriptional regulation, exhibits intrinsic transactivation activity, and may interact with EP300. ECD Protein, Human (sf9, His-GST) is the recombinant human-derived ECD protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Protein ecdysoneless homolog; hSGT1; ECD
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95905-1
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Expression Region
M1-N644
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Protein Length
Full Length of Isoform-1
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Molecular Weight
88-108 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ECD (extracellular domain) recombinant proteins are pivotal in various fields of biomedicine and biotechnology, serving as essential tools for understanding protein interactions, cellular signaling, and therapeutic development. The extracellular domain of proteins, which is the portion that interacts with the external environment, plays a crucial role in receptor activation, binding affinity, and specificity. Researchers have increasingly focused on the production and characterization of ECD recombinant proteins to investigate their functions and potential applications in drug development, vaccine design, and biomarker discovery.
The advancements in genetic engineering and recombinant DNA technology have facilitated the expression of ECDs in various host systems, enabling the production of these proteins in significant quantities. This has allowed for detailed structural and functional studies, providing insights into mechanisms of action and disease pathways. Furthermore, ECD recombinant proteins have been utilized in the development of therapeutic antibodies and fusion proteins, enhancing the efficacy of targeted treatments.
As the demand for personalized medicine and targeted therapies increases, research on ECD recombinant proteins continues to thrive, offering new opportunities for innovative approaches to treat diseases such as cancer, autoimmune disorders, and infectious diseases. Understanding the intricacies of ECD interactions can lead to breakthroughs in drug design, improving treatment outcomes for patients worldwide. Thus, the study of ECD recombinant proteins remains a dynamic and essential area of research within the life sciences.
