Analytical Data
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Gene name
CERK
- Application
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Alternative Names
LK4; hCERK; Lipid kinase 4; Acylsphingosine kinase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TCT0
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Expression Region
Ser128~Ser278
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Molecular Weight
20kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CERK (chitinase-like protein) is a member of the chitinase family and has gained attention in recent years due to its potential roles in plant immunity and development. Research indicates that CERK is involved in the recognition of chitin, a key component of fungal cell walls, thereby triggering defense mechanisms in plants against fungal pathogens. Its ability to mediate signaling pathways related to innate immunity has led to increased interest in understanding its structure and function. The reconstitution of CERK as a recombinant protein offers valuable insights into its biochemical properties and interactions with chitin and other cellular components. By employing modern biotechnological techniques, researchers aim to elucidate the precise mechanisms by which CERK activates plant defense responses, potentially paving the way for developing crops with enhanced resistance to diseases. Additionally, characterizing CERK can also provide fundamental knowledge about its evolutionary significance among chitinase family proteins. Overall, the study of recombinant CERK holds promise not only for advancing our understanding of plant-pathogen interactions but also for applying this knowledge to improve agricultural practices and crop resilience.
