Analytical Data
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基因名
MAN2B1
- Application
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别名
MANB; LAMAN; Lysosomal alpha-mannosidase; Lysosomal acid alpha-mannosidase; Mannosidase alpha-B
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00754
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表达区间
Ala883~Gly1011
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分子量
18kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAN2B1, or Mannosidase Alpha Class 2B Member 1, is an essential enzyme involved in the lysosomal breakdown of glycoproteins, specifically in the hydrolysis of mannose-rich oligosaccharides. Deficiency in MAN2B1 leads to a rare genetic disorder known as beta-mannosidosis, characterized by a range of neurological and developmental issues due to the accumulation of unprocessed glycoproteins. Research on MAN2B1 recombinant protein focuses on understanding its structure, function, and role in cellular metabolism, particularly in lysosomal storage diseases. By producing MAN2B1 in recombinant systems, scientists aim to elucidate its enzymatic activity and interactions with substrates or pharmacological agents, facilitating the development of potential enzyme replacement therapies. Additionally, studying the recombinant MAN2B1 protein can provide insight into the molecular mechanisms underlying glycan processing and the pathophysiology associated with its deficiencies, paving the way for innovative therapeutic strategies to alleviate the symptoms of beta-mannosidosis and related disorders. The exploration of MAN2B1 not only enhances our comprehension of lysosomal function but also underscores the importance of enzymatic balance in maintaining cellular health.
