Analytical Data
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Gene name
MAN2B1
- Application
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Alternative Names
MANB; LAMAN; Lysosomal alpha-mannosidase; Lysosomal acid alpha-mannosidase; Mannosidase alpha-B
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00754
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Expression Region
Ala883~Gly1011
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Molecular Weight
18kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAN2B1, or Mannosidase Alpha Class 2B Member 1, is an essential enzyme involved in the lysosomal breakdown of glycoproteins, specifically in the hydrolysis of mannose-rich oligosaccharides. Deficiency in MAN2B1 leads to a rare genetic disorder known as beta-mannosidosis, characterized by a range of neurological and developmental issues due to the accumulation of unprocessed glycoproteins. Research on MAN2B1 recombinant protein focuses on understanding its structure, function, and role in cellular metabolism, particularly in lysosomal storage diseases. By producing MAN2B1 in recombinant systems, scientists aim to elucidate its enzymatic activity and interactions with substrates or pharmacological agents, facilitating the development of potential enzyme replacement therapies. Additionally, studying the recombinant MAN2B1 protein can provide insight into the molecular mechanisms underlying glycan processing and the pathophysiology associated with its deficiencies, paving the way for innovative therapeutic strategies to alleviate the symptoms of beta-mannosidosis and related disorders. The exploration of MAN2B1 not only enhances our comprehension of lysosomal function but also underscores the importance of enzymatic balance in maintaining cellular health.
