Analytical Data
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Gene name
MARCKS
- Application
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Alternative Names
80K-L; MACS; MRACKS; PKCSL; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain
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Species
Human
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Source
E. coli
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Tag
Two N- s, His- & SUMO-
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29966
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Expression Region
Gly2~Glu332
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Molecular Weight
40kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MARCKS (Myristoylated Alanine-Rich C Kinase Substrate) is a key protein involved in various cellular processes, including cell motility, signaling, and cytoskeletal dynamics. Its significance lies in its ability to interact with membranes and cytoskeletal components, affecting processes such as cell adhesion and migration. The phosphorylation of MARCKS by protein kinase C (PKC) regulates its cellular functions, making it a critical component in the signaling cascades linked to growth factor responses and inflammation. Research has highlighted MARCKS's role in various pathological conditions, including cancer, where its expression levels and activity can influence tumor progression and metastasis. Additionally, MARCKS is implicated in neurological disorders due to its involvement in synaptic plasticity and neuronal development. Understanding the structural and functional aspects of MARCKS, particularly through the study of recombinant forms of the protein, can provide insights into its mechanistic roles and potential therapeutic targets. Recombinant MARCKS proteins are utilized for examining the interactions with other cellular molecules, facilitating the exploration of its functions in disease models. Overall, the investigation of MARCKS and its derivatives is crucial for unraveling the complex web of cellular signaling and offers promising avenues for therapeutic intervention in various diseases.
