Analytical Data
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Gene name
Periostin/OSF-2
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简介
Periostin/OSF-2 Protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin/OSF-2 Protein, Rat (HEK293, His) is the recombinant rat-derived Periostin/OSF-2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 44.48 ng/mL, corresponding to a specific activity is 2.248×104 units/mg. Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 44.48 ng/mL， corresponding to a specific activity is 2.248×104 units/mg.
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Alternative Names
Periostin; Periostin, osteoblast specific factor (Predicted), isoform CRA_a; Postn; Postn_predicted; Osteoblast Specific Factor 2
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Species
Rat
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
D3ZAF5
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Expression Region
N24-S800
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AA Sequence
NSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAICGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPSNEAWDNLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNCARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDELSSFRAAAITSDLLESLGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNKKDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIFREIIQPAEKSLHEKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTNEEREILIGDKNALQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYVKGVNETLLVNELKSKESDIMTTNGVIHVVDKLLYPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYTTKIITKLVEPKIKVIQGSLQPIIKTEGPAMTKIHIEGEPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLQKFLQKDTPAKKIQANKRVQGS
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Protein Length
Partial
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Molecular Weight
95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Periostin, also known as OSF-2 (osteoblast-specific factor 2), is an extracellular matrix protein primarily involved in bone and soft tissue remodeling. It plays a critical role in various physiological processes, including cell adhesion, migration, and differentiation, as well as in the healing of injuries and the maintenance of tissue integrity. Research has shown that periostin is overexpressed in several pathological conditions, such as cancer progression, tissue fibrosis, and chronic inflammatory diseases, underscoring its importance in both normal physiological and pathological contexts. The recombinant form of periostin has gained attention for its potential therapeutic applications, particularly in regenerative medicine and tissue engineering. Studies have demonstrated that periostin can enhance the proliferation and differentiation of various cell types, including fibroblasts and osteoblasts, suggesting its utility in promoting tissue repair and regeneration. Additionally, understanding the molecular mechanisms underlying periostin's functions could advance the development of targeted therapies for diseases associated with its dysregulation. The ongoing research focuses on characterizing the effects of recombinant periostin in different biological settings and exploring its potential for clinical applications, particularly in enhancing healing processes and improving outcomes in tissue repair.
