Analytical Data
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Gene name
Chondroitinase ABC
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简介
Chondroitinase ABC protein is an endolytic glycosaminoglycan lyase with broad specificity that effectively degrades polysaccharides such as chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, and smaller degree of hyaluronic acid. This enzyme activity is facilitated by β-elimination of the 1,4-hexosamine bond, resulting in unsaturated tetra- and disaccharides. Chondroitinase ABC Protein, P. vulgaris (His) is the recombinant Chondroitinase ABC protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Chondroitin sulfate ABC endolyase; Chondroitin ABC endoeliminase; Chondroitin ABC lyase I; Chondroitin sulfate ABC lyase I; ChS ABC lyase I; Chondroitinase ABC I; cABC I; Endochondroitinase ABC; INN: Condoliase
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P59807
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Expression Region
A25-P1021
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AA Sequence
ATSNPAFDPKNLMQSEIYHFAQNNPLADFSSDKNSILTLSDKRSIMGNQSLLWKWKGGSSFTLHKKLIVPTDKEASKAWGRSSTPVFSFWLYNEKPIDGYLTIDFGEKLISTSEAQAGFKVKLDFTGWRAVGVSLNNDLENREMTLNATNTSSDGTQDSIGRSLGAKVDSIRFKAPSNVSQGEIYIDRIMFSVDDARYQWSDYQVKTRLSEPEIQFHNVKPQLPVTPENLAAIDLIRQRLINEFVGGEKETNLALEENISKLKSDFDALNIHTLANGGTQGRHLITDKQIIIYQPENLNSQDKQLFDNYVILGNYTTLMFNISRAYVLEKDPTQKAQLKQMYLLMTKHLLDQGFVKGSALVTTHHWGYSSRWWYISTLLMSDALKEANLQTQVYDSLLWYSREFKSSFDMKVSADSSDLDYFNTLSRQHLALLLLEPDDQKRINLVNTFSHYITGALTQVPPGGKDGLRPDGTAWRHEGNYPGYSFPAFKNASQLIYLLRDTPFSVGESGWNNLKKAMVSAWIYSNPEVGLPLAGRHPFNSPSLKSVAQGYYWLAMSAKSSPDKTLASIYLAISDKTQNESTAIFGETITPASLPQGFYAFNGGAFGIHRWQDKMVTLKAYNTNVWSSEIYNKDNRYGRYQSHGVAQIVSNGSQLSQGYQQEGWDWNRMQGATTIHLPLKDLDSPKPHTLMQRGERGFSGTSSLEGQYGMMAFDLIYPANLERFDPNFTAKKSVLAADNHLIFIGSNINSSDKNKNVETTLFQHAITPTLNTLWINGQKIENMPYQTTLQQGDWLIDSNGNGYLITQAEKVNVSRQHQVSAENKNRQPTEGNFSSAWIDHSTRPKDASYEYMVFLDATPEKMGEMAQKFRENNGLYQVLRKDKDVHIILDKLSNVTGYAFYQPASIEDKWIKKVNKPAIVMTHRQKDTLIVSAVTPDLNMTRQKAATPVTINVTINGKWQSADKNSEVKYQVSGDNTELTFTSYFGIPQEIKLSPLP
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Protein Length
Full Length of Mature Protein
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Molecular Weight
110 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Chondroitinase ABC is an important enzyme derived from the bacterium *Proteus vulgaris*, known for its ability to degrade chondroitin sulfate, a key component of the extracellular matrix in vertebrates. This enzyme has garnered significant attention in biomedical research due to its potential therapeutic applications in the treatment of neurological disorders, particularly spinal cord injuries and other conditions characterized by axonal regeneration deficits. The degradation of chondroitin sulfate can help to alleviate the inhibitory environment surrounding damaged nerves, thereby promoting axonal growth and recovery. Recombinant technology has enabled the production of Chondroitinase ABC in sufficient quantities for detailed studies and therapeutic explorations. Recent research efforts have focused on optimizing the expression and purification processes to enhance the enzyme's bioactivity and stability. Additionally, studies are ongoing to better understand its mechanism of action, ideal dosing regimens, and potential side effects, paving the way for its inclusion in clinical settings. Given its biological significance and therapeutic promise, Chondroitinase ABC stands as a crucial subject in regenerative medicine and neurobiology, as scientists aim to unlock its full potential in facilitating neuronal repair and improving patient outcomes after nervous system injuries.
