Analytical Data
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基因名
CPT2
- Application
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别名
Carnitine O-palmitoyltransferase 2, mitochondrial; CPT2; Carnitine palmitoyltransferase II (CPT II); CPT1
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种属
Human
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表达系统
E. coli
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标签
N-10*His;C-Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23786
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表达区间
A209-S658
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氨基酸序列
AYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS
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蛋白长度
Partial
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分子量
58 kDa, based on SDS-PAGE under reducing conditions
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CPT2 (Carnitine Palmitoyltransferase II) is a crucial enzyme in mitochondrial fatty acid oxidation, facilitating the transport of long-chain fatty acids into the mitochondria for energy production. Mutations in the CPT2 gene can lead to CPT2 deficiency, a metabolic disorder characterized by impaired fatty acid metabolism, which can result in severe consequences including muscle weakness, myoglobinuria, and even life-threatening complications during periods of fasting or strenuous exercise. Research into recombinant CPT2 protein has gained importance for several reasons. Firstly, understanding the structure and function of CPT2 can provide insights into the molecular mechanisms underpinning fatty acid metabolism and its regulation. Secondly, the production of recombinant CPT2 protein allows scientists to investigate the effects of specific mutations, which can help elucidate the pathophysiology of CPT2 deficiency. Moreover, recombinant CPT2 can be utilized in developing gene therapy approaches and enzyme replacement therapies for affected patients. Recent advances in protein engineering and expression systems have facilitated the generation of high-yield, biologically active CPT2, thus enabling detailed biochemical and pharmacological studies. This research not only enhances our understanding of CPT2's role in energy metabolism but also opens avenues for potential therapeutic interventions for metabolic disorders linked to fatty acid metabolism. Overall, the study of recombinant CPT2 is pivotal in addressing the challenges associated with CPT2 deficiency and offers a promising framework for advancing treatments for metabolic diseases.
