Analytical Data
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Gene name
DIO1
- Application
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Alternative Names
TXDI1; Type-I 5'-deiodinase
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24389
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Expression Region
Thr31~Thr125
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Molecular Weight
30kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DIO1, or type 1 iodothyronine deiodinase, is a crucial enzyme involved in thyroid hormone metabolism, specifically in the activation and deactivation of thyroxine (T4) and triiodothyronine (T3). Its primary function is to convert T4 into the more active T3 hormone, which plays vital roles in regulating metabolism, growth, and development. Understanding the biochemical properties and mechanisms of DIO1 is significant for elucidating thyroid-related disorders, including hypothyroidism and hyperthyroidism. The use of recombinant DIO1 protein in research allows scientists to study its enzymatic activity, interaction with other molecules, and its role in various physiological processes. Advances in recombinant DNA technology have made it possible to produce DIO1 in sufficient quantities for detailed biochemical assays and structural studies, enabling researchers to explore the enzyme’s functionality in various cellular contexts. Investigating the structural and functional aspects of DIO1 can ultimately contribute to the development of therapeutic strategies for managing thyroid diseases, as well as providing insights into the hormonal regulation of metabolic pathways. Thus, the study of recombinant DIO1 protein is a pivotal area in endocrinology and metabolic research, with implications for health and disease management.
