Analytical Data
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基因名
Wnt3a Surrogate
- Application
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生物活性
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 23.41 ng/mL, corresponding to a specific activity is 4.27×104 units/mg. Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 23.41 ng/mL, corresponding to a specific activity is 4.27×104 units/mg.
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别名
Wnt3a Surrogate, Human (HEK293, N-hFc)
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种属
Human
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表达系统
HEK293
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标签
N-hFc
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纯度
Greater than 95% as determined by SDS-PAGE.
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分子量
70-75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Wnt3a is a crucial member of the Wnt signaling pathway, which plays a vital role in various biological processes, including embryonic development, tissue homeostasis, and stem cell regulation. The aberration of Wnt signaling is linked to numerous diseases, particularly cancer, making Wnt3a a significant target for therapeutic interventions. The study of Wnt3a has been advanced by the production of surrogate recombinant proteins, which serve as functional substitutes to study the biological effects of Wnt signaling in vitro and in vivo. These recombinant proteins can facilitate the understanding of Wnt3a's role in cell proliferation, differentiation, and migration, as well as its interactions with other signaling pathways. By using surrogate recombinant Wnt3a, researchers can explore the precise mechanisms by which Wnt3a influences cellular behavior and identify potential pathways for drug targeting. The development of high-yield expression systems and effective purification techniques has enabled researchers to produce these proteins in a cost-effective manner, thereby enhancing the study of Wnt3a in experimental models. Overall, the exploration of Wnt3a surrogate recombinant proteins not only deepens our understanding of the Wnt signaling pathway but also holds promise for the development of novel therapeutic strategies to combat diseases associated with dysregulated Wnt signaling.
