Analytical Data
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基因名
PDGF R alpha
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简介
PDGF R alpha protein is a tyrosine protein kinase receptor for PDGFA, PDGFB, and PDGFC that coordinates embryonic development, cell proliferation, survival, and chemotaxis. Their functions vary, promoting or inhibiting cell proliferation and migration depending on the situation. PDGF R alpha Protein, Rat (HEK293, His) is the recombinant rat-derived PDGF R alpha protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its ability to inhibit the biological activity of PDGF-AA using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 0.6932 μg/mL in the presence of 100 ng/mL recombinant human PDGF-AA, corresponding to a specific activity is 1.443×103 U/mg. Measured by its ability to inhibit the biological activity of PDGF-AA using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 0.6932 μg/mL in the presence of 100 ng/mL recombinant human PDGF-AA, corresponding to a specific activity is 1.443×103U/mg.
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别名
Platelet-derived growth factor receptor alpha; PDGFR-alpha; PDGFR-2; CD140a; PDGFRA
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种属
Rat
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
NP_036934.1
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表达区间
L24-E523
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氨基酸序列
LLLPSILPNENEKIVPLSSSFSLRCFGESEVSWQHPMSEEEDPNVEIRTEENNSSLFVTVLEVVNASAAHTGWYTCYYNHTQTEESEIEGRHIYIYVPDPDMAFVPLGMTDSLVIVEEDDSAIIPCLTTDPDTEVTLHNNGRLVPASYDSRQGFNGTFSVGPYICEATVRGRTFKTSEFNVYALKATSELNLEMDTRQTVYKAGETIVVTCAVFNNEVVDLQWTYPGEVRNKGITMLEEIKLPSIKLVYTLTVPKATVKDSGDYECAARQATKEVKEMKTVTISVHEKGFVQIRPTFGHLETVNLHQVREFVVEVQAYPTPRISWLKDNLTLIENLTEITTDVQRSQETRYQSKLKLIRAKEEDSGHYTIIVQNDDDMKSYTFELSTLVPASILELVDDHHGSGGGQTVRCTAEGTPLPNIEWMICKDIKKCNNDTSWTVLASNVSNIITEFHQRGRSTVEGRVSFAKVEETIAVRCLAKNDLGIGNRELKLVAPSLRSE
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蛋白长度
Partial
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分子量
70-115 kDa due to glycosylation
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Platelet-Derived Growth Factor Receptor Alpha (PDGFR-α) is a receptor tyrosine kinase that plays a pivotal role in cellular processes such as proliferation, survival, and migration, particularly in mesenchymal cells. Its activation by platelet-derived growth factors is essential for normal physiological processes, including wound healing and tissue repair. However, aberrant PDGFR-α signaling has been implicated in various pathological conditions, including fibrosis, atherosclerosis, and various types of cancer, prompting significant interest in this receptor as a therapeutic target. The generation of recombinant PDGFR-α proteins has allowed researchers to study the structure-function relationships of the receptor, investigate its signaling pathways, and develop potential inhibitors. This recombinant protein serves as a valuable tool in elucidating the molecular mechanisms underlying PDGFR-α function and its contributions to disease pathogenesis. Moreover, understanding the interactions between PDGFR-α and its ligands at the molecular level may provide insights into the development of novel therapeutic strategies to combat diseases associated with dysregulated PDGFR-α activity. Thus, ongoing research on PDGFR-α recombinant proteins is vital to advancing our knowledge of its biological significance and therapeutic potential.
