Analytical Data
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Gene name
Glial fibrillary acidic
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简介
Glial fibrillary acidic protein (GFAP) belongs to the class III intermediate filament family and serves as a cell-specific marker that is critical for distinguishing astrocytes from other glial cells during the development of the central nervous system. GFAP plays a key role in differentiating the identity of astrocytes, contributing to their unique characteristics. Glial fibrillary acidic Protein, Human (432a.a, His) is the recombinant human-derived Glial fibrillary acidic protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
rHuGlial fibrillary acidic protein/GFAP, His; Glial Fibrillary Acidic Protein; GFAP
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P14136
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Expression Region
M1-M432
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Protein Length
Full Length
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Molecular Weight
54.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Glial fibrillary acidic protein (GFAP) is a key intermediate filament protein predominantly expressed in astrocytes, a type of glial cell in the central nervous system (CNS). GFAP plays a critical role in maintaining the structural integrity and function of astrocytes, which are essential for various CNS functions including neurotransmitter regulation, ion homeostasis, and blood-brain barrier maintenance. Research into GFAP has gained significant attention due to its involvement in various neurological disorders, such as Alzheimer's disease, multiple sclerosis, and gliomas. As a marker of astrogliosis, elevated levels of GFAP are often associated with neuroinflammation and CNS injury, making it a valuable biomarker for diagnosing neurological conditions. Additionally, GFAP is crucial in studies related to neurodevelopment and neurodegeneration, as its expression is modulated in response to injury or disease states. The recombinant production of GFAP has enabled researchers to explore its biochemical properties, interactions with other cellular components, and potential therapeutic applications. Moreover, understanding GFAP's role at the molecular level can illuminate broader physiological processes within the CNS. Consequently, GFAP has become a focal point in neurobiological research, aiming to unravel the complex interplay between glial cells and neurons, and to develop potential strategies for treating neurodegenerative diseases and brain injuries. Overall, the study of GFAP not only enhances our understanding of astrocyte function but also paves the way for innovative approaches in the field of neuroscience.
