Analytical Data
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基因名
PLCh1
- Application
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别名
PLCL3; PLCeta1; Phospholipase C-Like 3; Phosphoinositide phospholipase C-eta-1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q4KWH8
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表达区间
Met1~Ala237
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分子量
32kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLCh1 (Phospholipase C-eta 1) is an important enzyme involved in the phosphoinositide signaling pathway, which plays a crucial role in various cellular processes, including cell proliferation, differentiation, and apoptosis. Unlike other isoforms of phospholipase C, PLCh1 is unique due to its specific expression patterns and regulatory mechanisms. Research into PLCh1 has gained momentum as its dysregulation has been linked to several pathophysiological conditions, including cancer and neurodegenerative diseases. Understanding the structure and function of PLCh1 is essential for deciphering its role in cellular signaling and its potential as a therapeutic target. Recent studies have focused on recombinant protein expression of PLCh1 to analyze its enzymatic activity, substrate specificity, and interactions with other signaling molecules. The availability of high-quality recombinant PLCh1 allows researchers to explore its biophysical characteristics, enzymatic kinetics, and functional implications in cellular contexts. This line of research not only contributes to the fundamental understanding of phosphoinositide signaling but also paves the way for developing novel therapeutic strategies aimed at diseases where PLCh1 modulation may offer significant benefits. Thus, the investigation of PLCh1 recombinantly expressed proteins represents a promising frontier in the fields of biochemistry and molecular biology, with implications for advancing clinical treatments and improving patient outcomes in various diseases.












