Analytical Data
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Gene name
HLCS
- Application
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Alternative Names
HCS; Biotin-[Proprionyl-Coenzyme A-Carboxylase(ATP-hydrolysing)]Ligase; Biotin apo-protein ligase Holocarboxylase synthetase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P50747
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Expression Region
Met1~Ser172
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Molecular Weight
33kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HLCS (Holocarboxylase Synthetase) is an essential enzyme responsible for the post-translational modification of biotin-dependent enzymes, enabling the covalent attachment of biotin to its target proteins. This process is crucial for a range of metabolic pathways, including fatty acid synthesis, amino acid metabolism, and gluconeogenesis. Mutations in the HLCS gene can lead to biotin deficiency, resulting in a variety of clinical symptoms, such as neurological disorders, skin rashes, and impaired development. Given its significance in maintaining metabolic homeostasis, research on HLCS and its recombinant proteins has garnered increasing interest. Scientists are focused on elucidating the enzymatic mechanisms of HLCS to better understand its role in biotin metabolism and its implications for human health. Additionally, the production of recombinant HLCS proteins offers valuable tools for studying biotinylation in vitro and in vivo, contributing to potential therapeutic developments for related metabolic disorders. This research not only expands our understanding of HLCS’s biological functions but also paves the way for novel approaches in treating metabolic diseases associated with biotin deficiency.
