Analytical Data
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基因名
EIF5A
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简介
The GSK-3 beta protein is an enzyme that plays a crucial role in multiple cellular processes, including cell proliferation, differentiation, and apoptosis. It regulates various signaling pathways and is involved in the development of several diseases, such as cancer and neurodegenerative disorders. GSK-3 beta is active in its unphosphorylated form and can phosphorylate a wide range of substrates to modulate cellular functions. EIF5A Protein, Human (His) is the recombinant human-derived EIF5A protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
Eukaryotic translation initiation factor 5A-1; eIF-5A1; Rev-binding factor; eIF-4D
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
NP_001961
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表达区间
M1-K154
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蛋白长度
Full Length
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分子量
19 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF5A, or eukaryotic translation initiation factor 5A, is a highly conserved protein that plays a critical role in cellular processes, particularly in translation and the regulation of gene expression. Its unique structure features an unexpected amino acid, hypusine, which is formed by the posttranslational modification of the lysine residue. This modification is crucial for EIF5A’s function in promoting the translation of specific mRNAs, especially those associated with cell growth and proliferation. Given its involvement in various cellular pathways, including stress responses, cell differentiation, and apoptosis, EIF5A has garnered significant interest in biomedical research. Studies have linked EIF5A to numerous diseases, including cancer, where its overexpression is often correlated with poor prognosis. Research has also revealed that EIF5A regulates the translation of proline-rich proteins, contributing to its role in cellular metabolism and response to environmental stressors. Recombination of the EIF5A protein allows scientists to explore its functional domains, investigate its interaction with ribosomal components, and identify its role in translational control under different physiological conditions. Additionally, understanding EIF5A's mechanisms can provide insights into therapeutic strategies, particularly for malignancies where its dysregulation contributes to tumorigenesis. The ongoing studies into EIF5A recombinants are crucial for elucidating the complexities of posttranslational modifications and their impact on protein functionality, thereby enhancing our understanding of essential cellular processes and paving the way for potential clinical applications.
