Analytical Data
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Gene name
HLA-A*02:01-B2M&P53 WT(HMTEVVRRC) Tetramer
- Application
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Alternative Names
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A140T913 (G25-T305)&P61769
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Expression Region
A0A140T913 (G25-T305)&P61769 (I21-M119)&HMTEVVRRC
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AA Sequence
GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEPSSQPT&IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM&HMTEVVRRC
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Molecular Weight
260-265 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The HLA-A*02:01-B2M&P53 WT (HMTEVVRRC) tetramer is a crucial tool in the study of immune responses, particularly in relation to cancer immunology. HLA-A*02:01 is one of the most prevalent human leukocyte antigen (HLA) alleles, playing a significant role in presenting peptide antigens to T cells, which are essential for immune surveillance and response. The p53 protein, known as a tumor suppressor involved in regulating the cell cycle and maintaining genomic stability, is frequently mutated in various cancers. The wild-type (WT) form of p53 presents specific epitopes, such as HMTEVVRRC, which can trigger strong T cell responses. By utilizing the tetramer approach, researchers can efficiently identify and isolate T cells recognizing the p53 epitope presented by HLA-A*02:01. This method enhances our understanding of T cell specificity and functionality in patients, particularly in the context of malignancies where p53 mutations are implicated. Studying this tetramer can also aid in the development of novel immunotherapies that harness the immune system's ability to target tumor cells effectively while minimizing adverse effects. Overall, the HLA-A*02:01-B2M&P53 WT tetramer serves as a valuable asset in exploring the intricacies of immune recognition in cancer and holds potential for advancing personalized medicine approaches in oncology.
