Analytical Data
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基因名
TAS1R1
- Application
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别名
TAS1-R1; TR1; GPR70; T1R1; gm148; G Protein-Coupled Receptor 70
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种属
Mouse
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99PG6
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表达区间
Trp20~Gln258
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分子量
30kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TAS1R1 is a key member of the sweet taste receptor family, playing a crucial role in the detection of sweet compounds and certain amino acids. As a G protein-coupled receptor (GPCR), TAS1R1 is primarily expressed in taste buds and is essential for mediating flavor perception. Research on TAS1R1 recombinant proteins has gained momentum due to their potential implications in food science, nutrition, and metabolic health. The ability to produce and characterize TAS1R1 recombinant proteins allows scientists to gain insight into the molecular mechanisms underlying taste perception and food preference. Furthermore, understanding the signaling pathways involved in TAS1R1 activation could have significant implications for developing strategies to combat obesity and diabetes, as taste perception is directly linked to dietary choices. Studies have demonstrated that TAS1R1 interacts with TAS1R2 to form a heterodimer that responds to various sweet stimuli, leading to an increase in intracellular calcium levels and subsequent activation of downstream signaling pathways. By employing techniques such as recombinant protein expression, purification, and functional assays, researchers aim to elucidate the structure-function relationship of TAS1R1 and its ligands. This research is critical for exploring how variations in TAS1R1 may influence individual taste sensitivity and preference, which in turn impacts dietary habits and health outcomes. In summary, the investigation of TAS1R1 recombinant proteins is a burgeoning field that promises to enhance our understanding of taste mechanisms and provide potential intervention strategies for health-related issues associated with diet.












