Analytical Data
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Gene name
Draxin
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简介
The Draxin protein is critical in commissural development in the spinal cord and forebrain, serving as a chemical pulse guidance protein for commissural axons. It inhibits dorsal spinal cord neurite outgrowth and acts as a Wnt signaling antagonist by interacting with LRP6, inhibiting cytosolic β-catenin stabilization. Draxin Protein, Human (HEK293, His) is the recombinant human-derived Draxin protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human Draxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human LRP6 (HY-P77990). The KD is 7.243 nM. Measured by its binding ability in a functional ELISA. Immobilized Human Draxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human LRP6 (HY-P77990). The KD is 7.243 nM.
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Alternative Names
Dorsal inhibitory axon guidance protein; Neucrin; C1orf187
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Species
Human
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q8NBI3
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Expression Region
G26-V349
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AA Sequence
GALAPGTPARNLPENHIDLPGPALWTPQASHHRRRGPGKKEWGPGLPSQAQDGAVVTATRQASRLPEAEGLLPEQSPAGLLQDKDLLLGLALPYPEKENRPPGWERTRKRSREHKRRRDRLRLHQGRALVRGPSSLMKKAELSEAQVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV
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Protein Length
Full Length of Mature Protein
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Molecular Weight
50-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Draxin is a recently identified protein that plays a critical role in neuronal development and regeneration. Initially discovered during studies focused on understanding the molecular mechanisms underlying spinal cord repair, Draxin has emerged as a significant player in the guidance of neural progenitor cells and axonal growth. Its expression is notably high in the developing nervous system, indicating its involvement in key processes such as cell migration and differentiation. Researchers have also noted its potential interaction with various signaling pathways, which may provide insights into its function in neural regeneration. The exploration of Draxin as a recombinant protein has garnered attention for its therapeutic implications, particularly in treating neurodegenerative diseases and spinal cord injuries. As scientists delve deeper into its structure and interactions, the potential to leverage Draxin's properties to enhance neuronal survival and promote functional recovery becomes increasingly promising. Understanding the precise mechanisms through which Draxin operates will not only illuminate fundamental aspects of neurobiology but also pave the way for novel strategies in regenerative medicine. This research is paramount as the increasing prevalence of neurological disorders calls for innovative solutions to restore lost functions and improve patient outcomes.
