Analytical Data
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Gene name
COL4a4
- Application
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Alternative Names
COL4-A4; CA44; Collagen Alpha-4(IV)chain; Collagen Of Basement Membrane,Alpha-4 Chain
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P53420
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Expression Region
Val1469~Ser1690
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Molecular Weight
28kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
COL4A4 is a gene that encodes a chain of type IV collagen, which is a crucial component of the basement membrane, providing structural support to various tissues and organs. Mutations in COL4A4 are linked to several renal disorders, particularly Alport syndrome, a genetic condition characterized by progressive kidney disease, hearing loss, and eye abnormalities. Research on recombinant COL4A4 protein is significant for understanding its functional roles in health and disease. By producing this protein through recombinant DNA technology, researchers can investigate the structure-function relationships of type IV collagen, explore the molecular mechanisms underlying related pathologies, and develop potential therapeutic strategies for disorders linked to COL4A4 mutations. Additionally, recombinant COL4A4 can serve as a valuable tool for studying the interactions between collagen and other extracellular matrix components, which is essential for tissue engineering and regenerative medicine applications. Understanding the properties of this collagen subtype in a controlled environment could provide insights into the development of targeted therapies, enhancing approaches for treating Alport syndrome and other related diseases.
