Analytical Data
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基因名
BirA
- Application
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别名
Bifunctional ligase/repressor BirA; birA; bioR; dhbB
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种属
E.coli
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表达系统
E. coli
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标签
N-6*His;N-MBP
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P06709
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表达区间
M1-K321
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氨基酸序列
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
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蛋白长度
Full Length
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分子量
64-77 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BirA is a biotin ligase enzyme that is pivotal in the study of protein labeling and tracking within biological systems. It facilitates the attachment of biotin to proteins in a process known as biotinylation, which allows researchers to investigate protein interactions, localization, and dynamics in living cells. The ability of BirA to selectively label proteins with biotin has led to the development of advanced methodologies, such as biotin-avidin pull-down assays and tethered interactions studies, enhancing our understanding of molecular complexes within various cellular contexts. Moreover, BirA’s utility extends to applications in synthetic biology and therapeutic development, where precise control over protein functionality is crucial. The exploration of BirA and its variants focuses on improving labeling efficiency, specificity, and minimal interference with the native functions of proteins. In recent years, advancements in genetic engineering and molecular design have enabled the optimization of BirA for a broader range of applications, making it a valuable tool in contemporary biochemical research. This ongoing research contributes to elucidating the complexities of cellular mechanisms and holds promise for innovations in biotechnology and medicine.
