Analytical Data
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Gene name
PARP3
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简介
The PARP3 protein is a mono-ADP ribosyltransferase that responds to DNA damage, focusing on mono-ADP ribosylation of proteins such as histone H2B, XRCC5, and XRCC6. PARP3 plays a key role in the DNA repair process, participating in precise nonhomologous end joining and inhibiting G-quadruplex structure. PARP3 Protein, Human (His, Strep) is the recombinant human-derived PARP3 protein, expressed by E. coli , with N-His, N-Strep II labeled tag.
- Application
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Alternative Names
Protein mono-ADP-ribosyltransferase PARP3; ARTD3; IRT1; ADPRT-3; PARP-3
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6F1-1
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Expression Region
M1-L533
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Protein Length
Full Length of Isoform-1
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Molecular Weight
63.3 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PARP3 (Poly(ADP-ribose) polymerase 3) is a member of the PARP protein family, which plays a crucial role in cellular responses to DNA damage. Unlike its more extensively studied counterpart, PARP1, PARP3 is less understood but has emerged as a significant player in the regulation of DNA repair processes, particularly in response to oxidative stress and chromosomal stability. PARP3 has been implicated in the PARylation of proteins involved in various cellular functions, including cell survival, differentiation, and apoptosis. Recent studies suggest that PARP3 interacts with several key proteins involved in DNA repair, including XRCC1 and BRCA1, indicating its potential involvement in both single-strand and double-strand break repair pathways. Given its unique structural features and functional roles, researchers are increasingly interested in investigating the biochemical properties of PARP3 through recombinant protein techniques. By producing PARP3 as a recombinantly expressed protein, scientists aim to elucidate its enzymatic activity, substrate specificity, and interaction dynamics with other proteins in the context of DNA repair pathways. Understanding the role of PARP3 in these mechanisms may reveal novel therapeutic targets in cancer treatment, particularly in tumors with defective DNA repair systems. As a result, PARP3's study is pivotal not only for basic biology but also for its therapeutic implications in oncology.
