Analytical Data
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Gene name
CK15
- Application
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Alternative Names
KRT15; K15, CK15, K1CO; KRTB; Keratin 15; Keratin-15,Basic; Keratin-15,Beta; Keratin, type I cytoskeletal 15
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19012
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Expression Region
Val274~Leu399
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Molecular Weight
16kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CK15, also known as cytokeratin 15, is a type of intermediate filament protein that plays a crucial role in the structural integrity of epithelial tissues, particularly in stratified and keratinizing epithelia. Its expression is predominantly observed in the epithelial cells of hair follicles, the cornea, and in certain types of cancers, such as squamous cell carcinoma. The study of CK15 recombinant protein has gained significance in recent years due to its potential applications in understanding epithelial cell differentiation, tissue regeneration, and cancer biology. Researchers have been particularly interested in elucidating the role of CK15 in maintaining the stemness of epithelial progenitor cells, as well as its involvement in the mechanical properties of tissues. As a recombinant protein, CK15 can be produced in vitro, allowing for more extensive studies on its biochemical properties and interactions with other cellular components. This has opened new avenues for investigating targeted therapies in cancer treatment and regenerative medicine, highlighting the need to develop robust methodologies for CK15 production, characterization, and functional assays. Overall, the research surrounding CK15 recombinant protein is pivotal for advancing our understanding of epithelial dynamics and for exploring new therapeutic strategies in tissue engineering and cancer treatment.
