Analytical Data
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Gene name
GUCY2C/Guanylyl cyclase C
- Application
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Alternative Names
Guanylyl cyclase C (GC-C) (Intestinal guanylate cyclase) (GUC2C) (STAR)
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Species
Human
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Source
HEK293
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Tag
N- His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P25092
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Expression Region
24-430aa
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Molecular Weight
49.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Guanylyl cyclase C (GC-C) is a receptor guanylyl cyclase that plays a crucial role in the regulation of fluid homeostasis, digestive function, and cell signaling. It is primarily activated by two ligands: guanylin and uroguanylin, which are secreted by intestinal cells. Upon activation, GC-C catalyzes the conversion of GTP to cyclic GMP (cGMP), a second messenger that triggers various physiological responses. Dysregulation of GC-C signaling has been implicated in several gastrointestinal disorders, including diarrhea and inflammatory bowel disease, making it a potential therapeutic target. Research on recombinant forms of GC-C, such as GUCY2C, has advanced our understanding of its structure and function, particularly in the context of ligand binding and downstream signaling pathways. Utilizing recombinant proteins allows for detailed biochemical studies, elucidating the molecular mechanisms underlying GC-C activity and its interactions with various signaling molecules. Understanding these processes has significant implications for developing novel treatments for related diseases, highlighting the importance of GC-C research in biomedicine.
