Analytical Data
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Gene name
KIF11
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简介
KIF11 Protein, a pivotal motor protein, orchestrates mitotic spindle formation and aids in Golgi-to-surface protein transport. Beyond mitosis, it engages in complex interactions, binding to thyroid hormone receptor, participating in chromatin remodeling, and interacting with NEK6, RARRES1, and AGBL2. This multifaceted role highlights KIF11's diverse contributions to cellular processes. KIF11 Protein, Human is the recombinant human-derived KIF11 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
KIF11; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52732
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Expression Region
M1-K368
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AA Sequence
MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVNQK
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Protein Length
Partial
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Molecular Weight
41 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KIF11, also known as kinesin-5 or Eg5, is a member of the kinesin superfamily of motor proteins, primarily involved in mitotic spindle assembly and chromosome segregation during cell division. As a motor protein that facilitates the movement of cellular components along microtubules, KIF11 plays a crucial role in maintaining proper cell division and is essential for the formation of the bipolar spindle. Dysregulation or overexpression of KIF11 has been implicated in various cancers, making it a significant target for therapeutic intervention. Researchers have focused on developing recombinant KIF11 protein for several purposes, including structural studies, functional assays, and drug screening. The recombinant form of KIF11 allows scientists to investigate its mechanistic role in mitosis and the potential effects of small-molecule inhibitors that can disrupt its function. By studying KIF11, researchers aim to better understand the molecular mechanisms underlying cell division and identify novel cancer treatment strategies. Furthermore, the availability of purified recombinant KIF11 protein enables high-throughput screening efforts to discover compounds that can selectively inhibit its activity, thus contributing to the development of targeted cancer therapies. Overall, the study of KIF11 and its recombinant protein holds significant promise for advancing cancer research and developing new therapeutic approaches.
