Analytical Data
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Gene name
acnB
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简介
The acnB protein coordinates short-chain fatty acid catabolism through the TCA pathway and 2-methylcitric acid cycle I, demonstrating catalytic versatility. It is involved in the reversible isomerization of citric acid to isocitrate and the hydration of 2-methyl-cis-aconitic acid. acnB Protein, E. coli is the recombinant Virus-derived acnB protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
Aconitate hydratase B; ACN; Aconitase; RNA-binding protein; acnB; yacI; yacJ
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Species
Virus
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P36683
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Expression Region
M1-V865
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Protein Length
Full Length
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Molecular Weight
95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of acnB recombinant protein is grounded in its critical role in bacterial metabolism, particularly in the context of energy production and metabolic pathways. AcnB, or aconitase, is an important enzyme that catalyzes the isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle, which is essential for aerobic respiration in many microorganisms. Research into acnB not only enhances our understanding of fundamental metabolic processes but also sheds light on the regulatory mechanisms that bacteria employ to adapt to varying environmental conditions. Moreover, dysfunctions or alterations in acnB expression have been linked to various pathogenic traits in bacteria, making it a potential target for therapeutic interventions. By investigating acnB recombinant proteins through techniques like gene cloning and expression in heterologous systems, researchers aim to characterize its enzymatic properties, structural features, and regulatory roles in metabolic pathways. These studies have significant implications for biotechnology, microbial ecology, and the development of antimicrobial agents, as they could lead to novel strategies for controlling bacterial growth in both industrial and clinical settings. The detailed understanding of acnB's function further contributes to the broader knowledge of metabolic engineering, aiding in the design of more efficient microbial strains for various applications in bioproduction and bioremediation.
