Analytical Data
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Gene name
RPRD1B
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简介
RPRD1B, is a necessary scaffolding protein that maintains genetic integrity by regulating resolution of R-loops at both the transcription termination and DNA double-strand break (DSB) repair levels. In endometrial cancers, RPRD1B accelerates cell cycle through up-regulating Cyclin D1, CDK4, and CDK6. In addition, RPRD1B enhancse transcription of CCND1 and promotes cell proliferation by interacting with RNA polymerase II. RPRD1B enhances the β-Catenin·TCF4 transcriptional activity in response to Wnt signaling[1][2][3][4]. RPRD1B Protein, Human (HEK293, N-His) is the recombinant human-derived RPRD1B protein, expressed by HEK293 , with N-6*His labeled tag.
- Application
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Alternative Names
Regulation of nuclear pre-mRNA domain-containing protein 1B; C20orf77; CREPT
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Species
Human
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Source
HEK293
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAH33629.1
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Expression Region
M1-D326
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AA Sequence
MSSFSESALEKKLSELSNSQHSVQTLSLWLIHHRKHAGPIVSVWHRELRKAKSNRKLTFLYLANDVIQNSKRKGPEFTREFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELIKALQDLENAASGDATVRQKIASLPQEVQDVSLLEKITDKEAAERLSKTVDEACLLLAEYNGRLAAELEDRRQLARMLVEYTQNQKDVLSEKEKKLEEYKQKLARVTQVRKELKSHIQSLPDLSLLPNVTGGLAPLPSAGDLFSTD
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Protein Length
Full Length
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Molecular Weight
37-42 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RPRD1B, or RNA Polymerase II Related Domain 1B, is a protein implicated in various cellular processes, particularly in the regulation of gene expression and RNA processing. Previous studies have suggested that RPRD1B plays a significant role in the recruitment of certain transcription factors and spliceosomal components, thereby influencing the alternative splicing of pre-mRNA. Its involvement in the phosphorylation and modification of RNA Polymerase II underscores its importance in transcriptional regulation. Additionally, RPRD1B has been linked to various cellular stress responses and may play a role in oncogenesis, as its dysregulation has been associated with several types of cancer. The study of RPRD1B is crucial for understanding the intricate mechanisms of transcriptional control and its implications in normal cell function and disease. Furthermore, given the rising interest in therapeutics targeting RNA processing mechanisms, RPRD1B presents a promising candidate for research aimed at developing novel treatment strategies for conditions characterized by aberrant gene expression. Understanding the structure, function, and interactions of RPRD1B could provide insights into its role in cellular homeostasis and highlight its potential as a biomarker or therapeutic target in cancer and other diseases. As a result, further elucidation of RPRD1B's molecular mechanisms and biological significance is essential for advancing our knowledge of gene regulation and identifying new avenues for intervention in transcription-related disorders.
