Analytical Data
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Gene name
Carbonic Anhydrase 14
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简介
Carbonic Anhydrase 14 Protein, Mouse (His) is an approximately 40.0 kDa mouse carbonic anhydrase 14 protein with a His-flag. Carbonic Anhydrase 14 is a type I membrane enzyme highly expressed in all parts of the central nervous system[1].
- Application
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Alternative Names
rMuCarbonic Anhydrase 14, His; Carbonic Anhydrase 14; Carbonate Dehydratase XIV; Carbonic Anhydrase XIV; CA14;
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Species
Mouse
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9WVT6
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Expression Region
A16-M290
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AA Sequence
HHHHHHADGGHHWTYEGPHGQDHWPTSYPECGGDAQSPINIQTDSVIFDPDLPAVQPHGYDQLGTEPLDLHNNGHTVQLSLPPTLHLGGLPRKYTAAQLHLHWGQRGSLEGSEHQINSEATAAELHVVHYDSQSYSSLSEAAQKPQGLAVLGILIEVGETENPAYDHILSRLHEIRYKDQKTSVPPFSVRELFPQQLEQFFRYNGSLTTPPCYQSVLWTVFNRRAQISMGQLEKLQETLSSTEEDPSEPLVQNYRVPQPLNQRTIFASFIQAGPLYTTGEM
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Protein Length
Extracellular Domain
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Molecular Weight
40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Carbonic Anhydrase 14 (CA14) is a member of the carbonic anhydrase family of enzymes, which catalyze the reversible hydration of carbon dioxide to bicarbonate and protons, playing a crucial role in various physiological processes, including respiration, acid-base balance, and electrolyte transport. Unlike other carbonic anhydrases, CA14 is primarily expressed in the brain and the gastrointestinal tract, suggesting distinct functions in these tissues. Research has shown that CA14 may be involved in neuroprotection, synaptic function, and the regulation of ion transport in neurons. Its unique expression pattern and potential physiological roles have drawn interest in understanding its structure-function relationship. Recombinant CA14 protein is pivotal for studying the enzyme's catalytic mechanisms, inhibitor binding, and its role in various diseases, including neurological disorders. By producing CA14 as a recombinant protein, researchers can investigate its enzymatic properties, assess its interactions with potential drug compounds, and explore its therapeutic prospects. Additionally, the study of CA14 can contribute to our understanding of not only carbonate chemistry in biological systems but also its implications in pathophysiological conditions, thereby expanding the scope of carbonic anhydrases in biomedical research.
