Analytical Data
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Gene name
Aconitase 2/ACO2
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简介
Aconitase 2 (ACO2) protein catalyzes the isomerization of citric acid to isocitrate through the intermediate aconitate, playing a crucial role in cellular metabolism. This enzyme activity is a key step in the tricarboxylic acid (TCA) cycle, a central pathway for energy production and generation of metabolic intermediates. Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Aconitate hydratase; Aconitase; ACO2
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99KI0
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Expression Region
Q28-Q780
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Protein Length
Full Length of Mature Protein
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Molecular Weight
100 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Aconitase 2 (ACO2) is a crucial enzyme involved in the citric acid cycle, primarily located in the mitochondria, and plays a significant role in cellular metabolism and energy production. The enzyme catalyzes the reversible conversion of citrate to isocitrate, facilitating crucial metabolic processes. Recent research has highlighted ACO2's involvement in cellular homeostasis and its regulation of iron metabolism, linking it to various physiological and pathological conditions, including neurodegenerative diseases and cancer. Understanding ACO2's structure and function is essential for deciphering its role in these conditions, which has prompted extensive studies on its recombinant protein form. Producing ACO2 as a recombinant protein allows for detailed biochemical and biophysical characterization, enabling researchers to investigate its enzymatic activity, regulatory mechanisms, and interactions with other biomolecules. Additionally, the availability of recombinant ACO2 opens avenues for potential therapeutic applications aimed at modulating its function in diseases associated with mitochondrial dysfunction. Thus, the study of ACO2 recombinant protein is not only pivotal for basic science but also holds promise for translational research aiming to develop targeted therapies for metabolic and oxidative stress-related disorders.
