Analytical Data
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Gene name
GGCT
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简介
The GGCT protein is a key player in sulfate assimilation, catalyzing the initial step in the conversion of inorganic sulfate and ATP to adenosine-5'-phosphate sulfate (APS). This activity is essential for the biosynthesis of sulfur-containing amino acids and is essential for cellular sulfur metabolism. GGCT Protein, Human (His) is the recombinant human-derived GGCT protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
rHuGamma-glutamylcyclotransferase/GGCT, His; Gamma-Glutamylcyclotransferase; Cytochrome C-Releasing Factor 21; GGCT; C7orf24; CRF21
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75223-1
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Expression Region
M1-L188
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AA Sequence
MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL
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Protein Length
Full Length of Isoform-1
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Molecular Weight
23.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of recombinant GGCT (gamma-glutamylcyclotransferase) proteins has garnered significant attention in the field of molecular biology and biochemistry due to their potential roles in various physiological processes and therapeutic applications. GGCT is an enzyme involved in the metabolism of glutathione and has been implicated in cellular stress responses, antioxidant defense mechanisms, and even neuroprotection. Its unique catalytic ability to convert gamma-glutamyl compounds into 5-oxoproline highlights its importance in regulating glutamate levels and maintaining cellular homeostasis. Recent research has focused on the gene encoding GGCT, uncovering insights into its expression patterns and functions in different tissues. Recombinant expression systems have been utilized to produce GGCT for further study, enabling detailed investigations into its biochemical properties and interactions with other cellular components. Moreover, understanding the structure-function relationship of GGCT can pave the way for the development of targeted therapies for conditions associated with oxidative stress, such as neurodegenerative diseases and cancer. As we continue to explore the functional versatility of GGCT, the potential applications of this enzyme in medicine and biotechnology become increasingly relevant, making it a focal point of contemporary research endeavors.
