Analytical Data
-
Gene name
CD45
-
简介
CD45/PTPRC Protein, Mouse (HEK 293, His) expresses in HEK293 with a His tag at the N-terminus. CD45 is a transmembrane glycoprotein, expressed on almost all haematopoietic cells except for mature erythrocytes. PTPRC is an essential regulator of T and B cell antigen receptor-mediated activation[1].
- Application
-
Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD45 is coated at 0.5 μg/mL (100 μL/well) can bind Recombinant Mouse Galectin-1 (HY-P74131). The ED50 for this effect is 0.7718-0.7935 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD45 is coated at 0.5 μg/mL (100 μL/well) can bind Recombinant Mouse Galectin-1 (HY-P74131). The ED50 for this effect is 0.7718 μg/mL.
-
Alternative Names
rMuReceptor-type tyrosine-protein phosphatase C/CD45, His; B220; CD45 antigen; CD45; CD45R; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C
-
Species
Mouse
-
Source
HEK293
-
Tag
C-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P06800
-
Expression Region
Q26-K566
-
AA Sequence
QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK
-
Protein Length
Extracellular Domain
-
Molecular Weight
120-160 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CD45, also known as leukocyte common antigen, is a crucial glycoprotein expressed on the surface of all nucleated hematopoietic cells. It plays a significant role in regulating T and B cell signaling, activation, and development, making it an essential component in the immune response. The study of CD45 has gained momentum due to its involvement in various diseases, including autoimmune disorders, cancers, and infectious diseases. Understanding the structure and function of CD45, especially its isoforms resulting from alternative splicing, is vital for elucidating its complex role in immune regulation. Recent advances in recombinant protein technology have enabled the production of CD45 isoforms, allowing researchers to investigate their specific biological functions and therapeutic potentials. Additionally, recombinant CD45 proteins are being explored for use in diagnostic assays and as potential targets for immunotherapy. Overall, the ongoing research on CD45 recombinant proteins holds promise for developing novel strategies to manipulate the immune system for better disease management.
