Analytical Data
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基因名
OBCAM/OPCML
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简介
OBCAM/OPCML Protein uniquely binds opioids, especially in the presence of acidic lipids, indicating a potential role in cellular interactions and signaling related to opioid binding. This dual affinity suggests involvement in cell-contact processes where opioids and acidic lipids are present, highlighting its significance in modulating cellular responses to external stimuli and contributing to opioid-related signaling pathways. OBCAM/OPCML Protein, Human (HEK293, His) is the recombinant human-derived OBCAM/OPCML protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human OBCAM at 1 μg/mL (100 μL/well) can bind biotinylated human LSAMP. The ED50 for this effect is 0.9464 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human OBCAM at 1 μg/mL (100 μL/well) can bind biotinylated human LSAMP. The ED50 for this effect is 0.9464 μg/mL.
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别名
Opioid-binding protein/cell adhesion molecule; OBCAM; OPCML; IGLON1
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q14982-1
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表达区间
G28-N322
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氨基酸序列
GVPVRSGDATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVN
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蛋白长度
Full Length of OPCML
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分子量
45-60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OBCAM (Oligodendrocyte-Bound Cell Adhesion Molecule) and OPCML (Oligodendrocyte Precursor Cell Market-Like) proteins are significant in the context of neurobiology and cell adhesion research. Both proteins are members of the immunoglobulin superfamily and have been implicated in crucial processes such as oligodendrocyte development, myelination, and neuronal-glial interactions. OBCAM, predominantly expressed in oligodendrocytes, plays a role in myelin sheath formation and maintenance, impacting the overall functionality of the central nervous system. Conversely, OPCML is associated with oligodendrocyte precursor cell regulation and may exert tumor suppressive functions in various cancers, suggesting its broader relevance beyond neurobiology. Research into these proteins has gained momentum due to their potential implications in neurodegenerative diseases, such as multiple sclerosis and amyotrophic lateral sclerosis, where myelin degeneration is a critical factor. Understanding the mechanisms underlying OBCAM and OPCML functions could lead to novel therapeutic strategies aimed at promoting remyelination and restoring neuronal health. Recent advances in recombinant protein technology have enabled the study of these molecules in greater detail, facilitating insights into their structure-function relationships and interactions with other cellular components. As the scientific community continues to explore the biological roles of OBCAM and OPCML, their study represents a promising avenue for advancing our knowledge of neural cell biology and developing targeted therapies for demyelinating conditions.
