Analytical Data
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Gene name
MDM4/MDMX
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简介
MDM4/MDMX proteins cooperate with MDM2 to regulate TP53 (p53) and inhibit the transcriptional activation domains of p53 and p73. This hinders their ability to induce cell cycle arrest and apoptosis. MDM4/MDMX Protein, Human (His) is the recombinant human-derived MDM4/MDMX protein, expressed by E. coli , with N-His labeled tag.
- Application
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Biological Activity
Measured by its ability to promote proliferation of A549 human non small cell lung cancer cell. The ED50 for this effect is 33.7 ng/mL, corresponding to a specific activity is 2.967×104 units/mg. Measured by its ability to promote proliferation of A549 human non small cell lung cancer cell. The ED50 for this effect is 33.7 ng/mL , corresponding to a specific activity is 2.967×104 units/mg.
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Alternative Names
Protein Mdm4; Protein Mdmx; MDM4; MDMX
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O15151-1
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Expression Region
M1-D134
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Protein Length
Partial
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Molecular Weight
19.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MDM4 (also known as MDMX) is a key regulatory protein that plays a crucial role in the modulation of p53, a well-known tumor suppressor involved in the cellular response to stress, DNA damage, and cell cycle regulation. Overexpression of MDM4 can inhibit p53's tumor-suppressive functions, contributing to the development and progression of various cancers. Research into the role of MDM4/MDMX has gained attention due to its potential as a therapeutic target in cancer treatment. The understanding of its structure and function is essential, as it can provide insights into how MDM4 interacts with p53 and other cellular pathways. Additionally, the development of recombinant MDM4/MDMX proteins allows researchers to explore their biochemical properties, interactions, and the mechanisms through which they modulate p53 activity. This research is pivotal in identifying new strategies for cancer therapy, especially in tumors characterized by p53 inactivation or mutation. Thus, studying the MDM4/MDMX protein not only enhances our understanding of cancer biology but also opens avenues for the development of novel therapeutic approaches aimed at restoring p53 function in malignancies.
