Analytical Data
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Gene name
PTP1B
- Application
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Alternative Names
Protein-tyrosine phosphatase 1B (PTP-1B) (PTP1B)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P18031
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Expression Region
1-321aa
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Molecular Weight
44.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTP1B, or protein tyrosine phosphatase 1B, is a crucial enzyme involved in the regulation of various signaling pathways, particularly those related to insulin and other growth factor receptor signaling. Since its discovery, PTP1B has gained significant attention due to its role in insulin signaling and glucose homeostasis, making it a potential therapeutic target for metabolic disorders, including type 2 diabetes and obesity. The study of PTP1B has revealed its localization in the endoplasmic reticulum and its involvement in the dephosphorylation of key tyrosine residues on insulin receptor substrates. This action negatively influences insulin signaling, thereby modulating glucose uptake and metabolic response. Furthermore, PTP1B has been implicated in the development of resistance to insulin, fostering an environment conducive to the onset of diabetes. Researchers have been focused on developing PTP1B inhibitors as a promising approach for enhancing insulin action and ameliorating insulin resistance. The generation of recombinant PTP1B protein has facilitated biochemical assays, structural studies, and high-throughput screening of potential inhibitors, advancing our understanding of its function and providing insights into novel therapeutic strategies. Overall, the research surrounding PTP1B and its recombinant protein forms has profound implications for the treatment of metabolic diseases, highlighting the importance of targeted interventions in signaling pathways.
