Analytical Data
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基因名
Maleylacetoacetate isomerase/GSTZ1
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简介
The maleylacetoacetate isomerase/GSTZ1 protein is a member of the GST superfamily and detoxifies electrophilic molecules. It converts maleylacetoacetate to fumarylacetoacetate, a key step in phenylalanine/tyrosine degradation. Maleylacetoacetate isomerase/GSTZ1 Protein, Human (N-His) is the recombinant human-derived Maleylacetoacetate isomerase/GSTZ1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
rHuMaleylacetoacetate isomerase/GSTZ1, His; Maleylacetoacetate Isomerase; MAAI; GSTZ1-1; Glutathione S-Transferase Zeta 1; GSTZ1
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
NP_665877.1
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表达区间
Q2-A216
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氨基酸序列
QAGKPILYSYFRSSCSWRVRIALALKGIDYETVPINLIKDGGQQFSKDFQALNPMKQVPTLKIDGITIHQSLAIIEYLEEMRPTPRLLPQDPKKRASVRMISDLIAGGIQPLQNLSVLKQVGEEMQLTWAQNAITCGFNALEQILQSTAGIYCVGDEVTMADLCLVPQVANAERFKVDLTPYPTISSINKRLLVLEAFQVSHPCRQPDTPTELRA
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蛋白长度
Full Length of Mature Protein
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分子量
25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Maleylacetoacetate isomerase (MAAi) is an enzyme that plays a crucial role in the catabolic pathway of tyrosine, facilitating the conversion of maleylacetoacetate into fumarate and acetoacetate. Deficiencies in MAAi can lead to metabolic disorders such as hereditary tyrosinemia, a condition characterized by the accumulation of toxic intermediates that can cause severe liver and kidney damage. The enzyme is encoded by the GSTZ1 gene, which also exhibits glutathione transferase activity, further implicating its importance in detoxification processes. Research on the recombinant protein of Maleylacetoacetate isomerase/GSTZ1 focuses on understanding its structural characteristics, catalytic mechanisms, and functional roles in metabolism. Detailed studies, including crystallography and kinetic analyses, aim to elucidate the interaction of MAAi with substrates and inhibitors, providing insights into potential therapeutic approaches for treating metabolic disorders. The recombinant expression of GSTZ1 facilitates its purification and characterization, enabling researchers to explore its enzymatic properties, stability, and the effects of genetic variations associated with human diseases. This research is essential for developing targeted treatments and understanding the biochemical pathways implicated in tyrosine metabolism, ultimately contributing to better diagnostics and therapeutic strategies for related metabolic disorders.
