Analytical Data
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Gene name
CDK5-p35
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简介
CDK5 is a proline-directed serine/threonine protein kinase that critically regulates neuronal cell cycle, differentiation and potential apoptosis in neuronal diseases by preventing cell cycle re-entry. It interacts with numerous proteins involved in neuronal development and coordinates processes such as survival, migration, differentiation, axonal growth, synaptogenesis, and neurotransmission. CDK5-p35 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK5-p35, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK5; cyclin-dependent kinase 5; PSSALRE; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit;
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00535-1 (M1-P292)&Q15078
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Expression Region
Q00535-1 (M1-P292)&Q15078 (G2-R307)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclin-dependent kinase 5 (CDK5) is a serine/threonine kinase that plays a crucial role in the regulation of neuronal development and function. Unlike other cyclin-dependent kinases, CDK5 is activated by specific regulatory proteins, specifically p35 and p39. The CDK5-p35 complex is critical for various cellular processes, including neurogenesis, synaptic plasticity, and the maintenance of neuronal architecture. Abnormal activation of CDK5 has been implicated in various neurodegenerative diseases, such as Alzheimer's disease, where the cleavage of p35 into p25 leads to hyperactivation of CDK5, contributing to neurotoxicity and neurofibrillary tangle formation. Research on recombinant CDK5-p35 proteins has gained significant interest as a means to understand the kinase's specific roles in neuronal signaling and pathology. This protein has been studied for its structural properties, enzymatic activity, and interactions with other proteins involved in cellular signaling pathways. By generating and analyzing recombinant CDK5-p35, researchers aim to delineate the precise mechanisms by which CDK5 influences neuronal health and its potential as a therapeutic target for neurodegenerative disorders. Understanding the CDK5-p35 pathway is critical, as it may reveal valuable insights into preventing or treating conditions characterized by neuronal dysfunction.
