Analytical Data
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Gene name
GFAP
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简介
The GFAP protein is a member of the class III intermediate filament family and serves as a cell-specific marker critical for distinguishing astrocytes from other glial cells in the developing central nervous system. Its role as a marker is critical in defining the identity of astrocytes, highlighting their unique characteristics in a complex cellular environment. GFAP Protein, Mouse (His-SUMO) is the recombinant mouse-derived GFAP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Application
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Alternative Names
Glial fibrillary acidic protein; GFAP
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Species
Mouse
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Source
E. coli
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Tag
N-6*His;N-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P03995
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Expression Region
M1-M430
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AA Sequence
MERRRITSARRSYASETVVRGLGPSRQLGTMPRFSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNFAQDLGTLRQKLQDETNLRLEAENNLAAYRQEADEATLARVDLERKVESLEEEIQFLRKIYEEEVRELREQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDAASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKDSKQEHKDVVM
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Protein Length
Full Length
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Molecular Weight
65.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Glial fibrillary acidic protein (GFAP) is a key intermediate filament protein predominantly expressed in astrocytes, a type of glial cell in the central nervous system. It plays a crucial role in maintaining the structural integrity and function of astrocytes, contributing to the blood-brain barrier, and participating in neuroinflammatory processes. Research into recombinant GFAP has gained momentum due to its potential significance in neurological disorders. Elevated GFAP levels in cerebrospinal fluid and serum are associated with various conditions such as Alzheimer's disease, multiple sclerosis, and traumatic brain injury, making it a promising biomarker for diagnosis and monitoring disease progression. The production of recombinant GFAP enables detailed studies of its molecular structure, interactions, and functional properties in vitro and in vivo, facilitating the exploration of its role in astrocyte activation and neuroinflammation. Additionally, recombinant GFAP can be utilized in therapeutic approaches and drug development, as it may help elucidate the mechanisms underlying astrocyte-related pathologies. Overall, the study of GFAP and its recombinant forms is vital for advancing our understanding of glial cell biology and its implications in health and disease.
