Analytical Data
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Gene name
PLK1
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简介
PLK1 Protein, the subject of the paragraph, is not mentioned in the provided text.If you would like a summary involving PLK1, please provide the relevant paragraph or information about PLK1.PLK1 Protein, Mouse (sf9, His) is the recombinant mouse-derived PLK1 protein, expressed by Sf9 insect cells, with N-His labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase PLK1; PLK-1; STPK13
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Species
Mouse
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q07832
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Expression Region
M1-S603
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Protein Length
Full Length
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PLK1 (Polo-like kinase 1) is a crucial serine/threonine kinase involved in cell cycle regulation, particularly during mitosis. Its primary functions include the initiation of mitotic entry, centrosome maturation, and the orchestration of spindle assembly. Aberrant expression or activity of PLK1 has been implicated in various cancers, making it a promising target for cancer therapy. Research into PLK1 has revealed its role not only in the regulation of cell division but also in processes such as DNA damage repair and the maintenance of genomic stability. The production of recombinant PLK1 protein has become essential for elucidating its mechanistic roles and interactions in vitro and in vivo. Moreover, studying the biochemical properties of PLK1 is crucial for the development of specific inhibitors that could potentially serve as effective anticancer agents. This line of research has been propelled by the increasing understanding of cellular signaling pathways and the importance of precise kinase activity in maintaining cellular homeostasis. Given these factors, recombinant PLK1 proteins are critical for advancing our knowledge of its biological functions and for drug development efforts aimed at targeting PLK1 in cancer therapies.
