Analytical Data
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基因名
PLK1
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简介
PLK1 is a serine/threonine protein kinase that centrally coordinates key functions during the M phase of the cell cycle. It regulates centrosome maturation, spindle assembly, cohesin removal, inactivates APC/C inhibitors, and controls mitotic exit and cytokinesis. PLK1 Protein, Human (sf9, His) is the recombinant human-derived PLK1 protein, expressed by Sf9 insect cells , with N-10*His labeled tag.
- Application
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别名
Serine/threonine-protein kinase PLK1; PLK-1; STPK13
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种属
Human
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表达系统
Baculovirus
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标签
N-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P53350
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表达区间
M1-S603
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蛋白长度
Full Length
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分子量
66 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLK1 (Polo-like kinase 1) is a crucial serine/threonine kinase involved in cell cycle regulation, particularly during mitosis. Its primary functions include the initiation of mitotic entry, centrosome maturation, and the orchestration of spindle assembly. Aberrant expression or activity of PLK1 has been implicated in various cancers, making it a promising target for cancer therapy. Research into PLK1 has revealed its role not only in the regulation of cell division but also in processes such as DNA damage repair and the maintenance of genomic stability. The production of recombinant PLK1 protein has become essential for elucidating its mechanistic roles and interactions in vitro and in vivo. Moreover, studying the biochemical properties of PLK1 is crucial for the development of specific inhibitors that could potentially serve as effective anticancer agents. This line of research has been propelled by the increasing understanding of cellular signaling pathways and the importance of precise kinase activity in maintaining cellular homeostasis. Given these factors, recombinant PLK1 proteins are critical for advancing our knowledge of its biological functions and for drug development efforts aimed at targeting PLK1 in cancer therapies.
