Analytical Data
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Gene name
MRCKβ
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简介
CDC42BPB protein is a key serine/threonine protein kinase downstream of CDC42 that controls cytoskeletal dynamics and cell migration. CDC42BPB phosphorylates PPP1R12C and MYL9/MLC2, regulates actin reorganization and shapes cell dynamics. MRCKβ Protein, Human (sf9, GST) is the recombinant human-derived MRCKβ, expressed by Sf9 insect cells, with GST labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y5S2
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Expression Region
S2-G473
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MRCKβ (Myotonic Dystrophy Kinase Related Cdc42-Binding Kinase β) is a serine/threonine kinase that belongs to the AGC family of kinases and plays a pivotal role in various cellular processes, including cytoskeletal organization, cell migration, and cell proliferation. Its involvement in signal transduction pathways makes MRCKβ a crucial regulator of tumorigenesis and metastasis, particularly in cancer types where cell motility is a key factor. The study of MRCKβ is gaining momentum as researchers seek to understand its functional mechanisms and regulatory networks. Abnormal expression or activity of MRCKβ has been implicated in several diseases, notably cancer, where it may contribute to aggressive phenotypes and poor patient prognosis. Moreover, recent findings suggest that MRCKβ might interact with other signaling molecules such as Rho GTPases, intensifying its role in regulating the actin cytoskeleton and facilitating cancer cell invasion. This has prompted investigations into its potential as a therapeutic target, as inhibiting MRCKβ could diminish the invasive capabilities of cancer cells. Overall, research surrounding MRCKβ recombinant proteins aims to elucidate its biological functions, explore its molecular interactions, and assess its potential in targeted therapies, presenting an exciting frontier in cancer biology and treatment strategies.
