Analytical Data
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Gene name
PASK
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简介
PASK is an important serine/threonine protein kinase that complexly regulates energy homeostasis and protein translation. It phosphorylates key targets (EEF1A1, GYS1, PDX1, RPS6) in response to changing environmental conditions (e.g., oxygen levels and nutritional status). PASK Protein, Human (sf9, GST) is the recombinant human-derived PASK protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
PASK; PAS domain-containing serine/threonine-protein kinase; PAS-kinase; PASKIN; hPASK
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96RG2
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Expression Region
S949-S1323
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PASK, or PAS kinase, is a serine/threonine kinase that plays a crucial role in various cellular processes, including metabolism, stress response, and energy homeostasis. Its unique structure features a PAS (Per-ARNT-Sim) domain that enables it to sense environmental changes, thereby modulating downstream signaling pathways. Research has indicated that PASK is involved in the regulation of glucose and lipid metabolism, linking it to diseases such as obesity and diabetes. The study of PASK recombinant proteins has gained traction as researchers aim to elucidate its functional mechanisms and interactions with other cellular components. Recent advances in recombinant DNA technology and protein expression systems have facilitated the production of PASK proteins in significant quantities. This enables detailed functional assays, structural analyses, and characterization of PASK's enzymatic activities. By investigating the structure-function relationship of PASK, scientists seek to uncover its precise role in metabolic regulation and its potential as a therapeutic target for metabolic disorders. The ongoing research into PASK and its recombinant forms holds promise for developing novel strategies to manipulate metabolic pathways and combat related diseases, thereby contributing to the field of metabolic health and therapeutics.
