Analytical Data
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基因名
DENV2 Envelope
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简介
NS1 Protein, essential in virus budding, binds the cell membrane, forming a nucleocapsid that becomes the virus core. During entry, NS1 facilitates viral genome penetration post-hemifusion. NS1 migrates to the nucleus, influencing host functions, and counteracts host EXOC1's antiviral effects by sequestering and degrading EXOC1 via the proteasome pathway. Additionally, NS1 impedes RNA silencing by interfering with the host Dicer enzyme. DENV2 Envelope Protein (HEK293, His) is the recombinant DENV2 Envelope protein, expressed by HEK293, with N-His labeled tag.
- Application
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别名
Envelope Protein/E Protein (DENV); LGenome polyprotein; L
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种属
Virus
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表达系统
HEK293
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P29990
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表达区间
M281-S677
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蛋白长度
Partial
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分子量
55-60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Dengue virus (DENV) is a significant global health concern, responsible for millions of infections each year, particularly in tropical and subtropical regions. The virus exists in four serotypes, with DENV2 being one of the most widely researched due to its association with severe dengue cases. The envelope (E) protein of DENV is a key component in the virus's structure, playing a crucial role in viral entry into host cells and eliciting immune responses. Research on the recombinant Envelope protein from DENV2 has gained attention for its potential applications in vaccine development and diagnostics. By expressing the E protein in various systems, scientists aim to understand its immunogenic properties and determine how it interacts with host immune mechanisms. Furthermore, recombinant E proteins can be used to generate specific antibodies, which are essential for both therapeutic interventions and the development of serological assays. Investigating the structural and functional characteristics of the DENV2 Envelope protein can provide insights into the virus's pathogenesis and aid in the design of effective vaccines that elicit robust immune responses without causing disease enhancement. This research is critical in combating dengue fever and reducing the burden of this disease worldwide.
