Analytical Data
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Gene name
hERG
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简介
The hERG protein is the α subunit of voltage-gated potassium channels and is critical for mediating cardiac delayed rectifier potassium current (IKr). It lacks individual channel activity but dynamically affects properties by forming heterotetramers with other isoforms. hERG Protein, Human (HEK293) is the recombinant human-derived hERG protein, expressed by HEK293, with tag free.
- Application
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Species
Human
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12809-1
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Expression Region
M1-S1159, ΔP141-A350 and ΔS871-R1005
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Protein Length
Full Length of Isoform-1
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The human ether-à-go-go-related gene (hERG) encodes a potassium ion channel that plays a critical role in cardiac repolarization. Dysfunction or impairment of hERG channels can lead to severe cardiac arrhythmias, including long QT syndrome and torsades de pointes, conditions that can result in sudden cardiac death. Due to its importance in cardiac physiology and its role in drug-induced cardiotoxicity, hERG has become a focal point in pharmacological studies. Specifically, many drugs can block hERG channels, leading to undesirable side effects. Therefore, research on hERG recombinant proteins has gained significance in the fields of cardiology and pharmacology. Scientists employ recombinant hERG proteins to investigate their biochemical properties, understand the molecular mechanisms underlying channel function, and screen potential drug candidates for hERG interactions. The expression of hERG as a recombinant protein allows for detailed studies on ion channel kinetics, pharmacodynamics, and the effects of mutations that may cause channel malfunction. As drug development progresses, understanding hERG channel behavior can help identify safer therapeutic options and minimize the risks associated with drug-induced arrhythmias. Consequently, the study of hERG recombinant proteins not only enhances our understanding of cardiac physiology but also contributes significantly to the development of safer medications in clinical practice.
