Analytical Data
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Gene name
SPHK1/Sphingosine Kinase 1
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简介
The SPHK1/sphingosine kinase 1 protein is a multifunctional lipid mediator that phosphorylates sphingosine to generate sphingosine-1-phosphate (SPP). SPHK1/Sphingosine Kinase 1 Protein, Human (sf9, His-GST) is the recombinant human-derived SPHK1/Sphingosine Kinase 1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Sphingosine kinase 1; SPK 1; SPHK1; SPK
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NYA1
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Expression Region
M1-L384
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Protein Length
Full Length
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Molecular Weight
64 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sphingosine Kinase 1 (SPHK1) is a pivotal enzyme in sphingolipid metabolism, responsible for phosphorylating sphingosine to form sphingosine-1-phosphate (S1P), a bioactive lipid that plays critical roles in various cellular processes such as proliferation, survival, migration, and immune responses. Dysregulation of SPHK1 and the consequent alterations in S1P levels have been implicated in numerous pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders. Given the importance of SPHK1 in these processes, recombinant SPHK1 protein has emerged as a valuable tool for studying sphingolipid signaling pathways and for developing potential therapeutic strategies. Research utilizing this recombinant protein allows for the characterization of SPHK1 enzymatic activity, substrate specificity, and its interactions with other cellular components in vitro. Furthermore, it enables the exploration of the enzyme's role in disease mechanisms and the assessment of small molecule inhibitors targeting SPHK1, which could lead to novel treatments for diseases associated with aberrant sphingolipid signaling. Thus, the study of recombinant SPHK1 not only enhances our understanding of sphingolipid biology but also contributes to the identification of therapeutic targets in various diseases.
