Analytical Data
-
基因名
Semaphorin-4A/SEMA4A
-
简介
Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 and is critical for intercellular signaling.It regulates synaptic development, promoting inhibitory synapses via PLXNB1 and excitatory synapses via PLXNB2.Semaphorin-4A/SEMA4A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
生物活性
Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED50 for this effect is 0.2-0.8 μg/mL. Immobilized Mouse Semaphorin 4A at 2 μg/mL (100 μL/well) can bind Biotinylated Human ILT4 protein. The ED50 for this effect is 0.6023 μg/mL.
-
别名
Semaphorin-4A; Semaphorin-B; Sema B; Sema4a; Semab; SemB
-
种属
Mouse
-
表达系统
HEK293
-
标签
C-6*His
-
纯度
Greater than 95% as determined by SDS-PAGE.
-
蛋白编号
Q62178
-
表达区间
T33-H682
-
氨基酸序列
TGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPH
-
蛋白长度
Extracellular Domain
-
分子量
67-95 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Semaphorin-4A (SEMA4A) is a member of the semaphorin family of proteins, which are known to play critical roles in various biological processes, including neuronal development, immune responses, and cancer progression. This protein acts primarily as a guidance cue during axon growth and neuronal connectivity, facilitating the proper formation of neural circuits. Recent research has highlighted the involvement of SEMA4A in immune system regulation, where it influences T cell activation and differentiation, suggesting its potential role as a therapeutic target in autoimmune diseases and cancers. Due to its diverse functional implications, recombinant SEMA4A proteins have become valuable tools for functional studies and therapeutic applications. Scientists are investigating the molecular mechanisms through which SEMA4A exerts its effects on cell signaling pathways, aiming to understand its role in promoting or inhibiting cellular responses. By producing and characterizing recombinant SEMA4A, researchers hope to elucidate its biological activities and interactions with specific receptors, contributing to the development of novel strategies for manipulating immune responses and enhancing neuronal repair mechanisms. This line of research is particularly relevant in the context of neurodegenerative diseases and cancer therapy, where modulating SEMA4A activity could lead to innovative treatment options. Overall, the study of recombinant SEMA4A holds promise for advancing our understanding of its biological functions and potential therapeutic applications in various fields of medicine.
