Analytical Data
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Gene name
MCR-1
- Application
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Alternative Names
Polymyxin resistance protein MCR-1
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A0R6L508
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Expression Region
1-541aa
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Molecular Weight
76.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCR-1 (Mobile Colistin Resistance-1) is a gene that confers resistance to colistin, an antibiotic often used as a last resort for treating multi-drug resistant infections, particularly those caused by Gram-negative bacteria. Discovered in 2015, MCR-1 raised significant global health concerns as it is located on plasmids, allowing its rapid dissemination among various bacterial species. The emergence of MCR-1 indicates a worrying trend toward the increasing prevalence of antibiotic resistance, complicating treatment protocols and threatening effective infection control. Researchers have focused on this resistance mechanism to understand its genetic basis, transmission dynamics, and its potential impact on public health. Investigating MCR-1 also involves studying its interaction with other resistance genes and the overall resistance patterns in bacterial populations. Moreover, efforts are being made to devise new antimicrobial strategies to combat MCR-1 carrying organisms and to monitor the spread of this gene in clinical and environmental settings. The urgency of this research is underscored by the rising incidence of infections caused by resistant pathogens, necessitating comprehensive surveillance and innovative therapeutic approaches to safeguard public health against the growing threat of antimicrobial resistance.
