Analytical Data
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Gene name
T4 DNA Polymerase
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简介
T4 DNA Polymerase, a pivotal enzyme in viral DNA replication, performs dual functions crucial for genome synthesis. As a polymerase, it synthesizes DNA, and with its exonucleolytic activity, it proofreads by degrading single-stranded DNA in the 3'- to 5'-direction, ensuring the accuracy and fidelity of replicated viral genomic DNA. T4 DNA Polymerase Protein, T4 phage (His) is the recombinant T4 DNA Polymerase protein, expressed by E. coli , with N-6*His labeled tag. T4 DNA Polymerase Protein, T4 phage (His), has molecular weight of ~103.6 kDa.
- Application
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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AA Sequence
MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMILGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSDEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNDEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGVGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDHSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG
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Molecular Weight
103.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
T4 DNA Polymerase is a key enzyme originally derived from the T4 bacteriophage, and it plays a pivotal role in DNA replication and repair processes. Its high fidelity and versatility make it an essential tool in various molecular biology applications, including PCR (Polymerase Chain Reaction), DNA sequencing, and cloning. Researchers have sought to produce recombinant forms of T4 DNA Polymerase to enhance its properties and applicability in laboratory settings. The recombinant production allows for the purification of the enzyme in larger quantities and with greater consistency, which is crucial for experimental reproducibility. Additionally, modifications to the enzyme can be engineered to improve stability, increase processivity, or expand substrate specificity. As a result, recombinant T4 DNA Polymerase has been widely adopted in genomics, synthetic biology, and biotechnology research, providing significant insights into DNA biochemistry and enabling advancements in genetic engineering and therapeutics. Ongoing studies continue to focus on optimizing the enzyme’s characteristics, exploring its structural biology, and understanding its interactions with various nucleic acids, which could lead to innovative applications in diagnostics and life sciences.
