Analytical Data
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基因名
PRMT3
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简介
PRMT3 protein is a multifunctional protein arginine N-methyltransferase. As a type I methyltransferase, it catalyzes monomethylation and asymmetric dimethylation of arginine residues in target proteins. In addition to its methyltransferase role, PRMT3 may also regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. PRMT3 Protein, Human (His) is the recombinant human-derived PRMT3 protein, expressed by E. coli , with N-10*His labeled tag.
- Application
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别名
Protein arginine N-methyltransferase 3; PRMT3; HRMT1L3
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种属
Human
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表达系统
E. coli
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标签
N-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O60678-1
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表达区间
C2-Q531
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氨基酸序列
CSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ
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蛋白长度
Full Length of Isoform-1
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分子量
63 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRMT3 (Protein Arginine Methyltransferase 3) is a member of the protein arginine methyltransferase family, which is involved in the post-translational modification of proteins through methylation of arginine residues. This modification plays a critical role in various biological processes, including gene expression regulation, signal transduction, and RNA processing. Research into PRMT3 has garnered interest due to its essential role in cellular function and its potential links to diseases such as cancer and neurodegenerative disorders. Specifically, PRMT3 is known to methylate several key proteins, including transcription factors and components of the spliceosome, thereby influencing cellular pathways. The study of recombinant PRMT3 protein provides insights into its catalytic activity, substrate specificity, and mechanisms of action. Understanding the structure and function of PRMT3 through recombinant protein technology can facilitate the development of targeted therapeutic strategies and help elucidate the contribution of arginine methylation to disease mechanisms. Furthermore, recombinant PRMT3 serves as a valuable tool for studying its biological roles and interactions in vitro, paving the way for future investigations into its implications in health and disease.
