Analytical Data
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基因名
Calreticulin/CALR
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简介
The calreticulin/CALR protein is a calcium-binding molecular chaperone that promotes ER folding and quality control. It interacts with monoglucosylated glycoproteins and promotes nuclear export of NR3C1. Calreticulin/CALR Protein, Human (His) is the recombinant human-derived Calreticulin/CALR protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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生物活性
When Recombinant Human CALR is immobilized at 2.5 µg/mL(100 µL/well) can bind Anti-CALR Antibody. The ED50 for this effect is <15 ng/mL. When Recombinant Human CALR is immobilized at 2.5 µg/mL(100 µL/well) can bind Anti-CALR Antibody. The ED50 for this effect is 5.221 ng/mL.
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P27797
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表达区间
E18-L417
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氨基酸序列
EPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL
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蛋白长度
Full Length of Mature Protein
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分子量
54 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The IAPP (Islet Amyloid Polypeptide), also known as amylin, is a peptide hormone co-secreted with insulin from the pancreatic beta cells. In humans, IAPP plays a crucial role in glucose metabolism and is involved in regulating appetite and gastric emptying. However, under pathological conditions, such as in type 2 diabetes, IAPP tends to misfold and aggregate, leading to the formation of amyloid plaques in the pancreas, which can contribute to beta cell dysfunction and insulin resistance. The study of IAPP and its amyloidogenic properties has garnered significant attention due to its implications in diabetes and metabolic disorders. Researchers focus on understanding the mechanisms underlying IAPP aggregation and toxicity, as well as exploring potential therapeutic strategies, including the development of inhibitors that prevent IAPP aggregation or promote its proper folding. Moreover, the exploration of IAPP’s structure-function relationship is essential for designing effective drugs aimed at mitigating its pathological effects. The reconstitution of IAPP proteins in vitro allows for detailed examination of their biophysical properties and interactions, paving the way for innovative therapeutic approaches to combat diabetes and improve metabolic health. Overall, the multifaceted role of IAPP in both physiology and pathology highlights the importance of ongoing research aimed at unraveling its complexities and potential as a target for treatment.
