Analytical Data
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基因名
Phytanoyl-CoA dioxygenase
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简介
Phytyl-CoA dioxygenase (PHYH) is an enzyme involved in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. PHYH catalyzes the conversion of phytyl-CoA to 2-hydroxyphytyl-CoA, initiating the degradation pathway. Phytanoyl-CoA dioxygenase Protein, Actinophytocola oryzae (His) is the recombinant Phytanoyl-CoA dioxygenase protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
Ectoine hydroxylase
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种属
Others
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A0A4R7W2E1
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表达区间
M1-F266
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Phytanoyl-CoA dioxygenase (Phytanoyl-CoA DIOX) is an essential enzyme in the catabolism of phytanic acid, a branched-chain fatty acid derived from the diet, particularly from ruminant animal products and certain plant sources. Deficiencies in this enzyme can lead to Refsum disease, a rare neurodegenerative disorder characterized by the accumulation of phytanic acid in tissues, resulting in a range of neurological symptoms. Understanding the structural and functional properties of Phytanoyl-CoA DIOX is crucial for elucidating its role in fatty acid metabolism and potential therapeutic strategies for Refsum disease. To investigate these aspects, researchers have employed recombinant DNA technology to produce the enzyme in a heterologous system, allowing for large-scale purification and characterization. The expression of the recombinant protein provides insights into substrate specificity, catalytic mechanisms, and potential inhibitors. By using techniques such as X-ray crystallography and molecular modeling, scientists aim to gain a detailed understanding of the enzyme's structure-function relationships, which can inform drug development and therapeutic interventions. This research not only sheds light on the molecular basis of metabolic disorders associated with phytanic acid but also contributes to the broader field of biochemistry and metabolic engineering.
