Analytical Data
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Gene name
Phytanoyl-CoA dioxygenase
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简介
Phytyl-CoA dioxygenase (PHYH) is an enzyme involved in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. PHYH catalyzes the conversion of phytyl-CoA to 2-hydroxyphytyl-CoA, initiating the degradation pathway. Phytanoyl-CoA dioxygenase Protein, Actinophytocola oryzae is the recombinant Phytanoyl-CoA dioxygenase protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
Ectoine hydroxylase
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Species
Others
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A4R7W2E1
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Expression Region
M1-F266
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Phytanoyl-CoA dioxygenase (Phytanoyl-CoA DIOX) is an essential enzyme in the catabolism of phytanic acid, a branched-chain fatty acid derived from the diet, particularly from ruminant animal products and certain plant sources. Deficiencies in this enzyme can lead to Refsum disease, a rare neurodegenerative disorder characterized by the accumulation of phytanic acid in tissues, resulting in a range of neurological symptoms. Understanding the structural and functional properties of Phytanoyl-CoA DIOX is crucial for elucidating its role in fatty acid metabolism and potential therapeutic strategies for Refsum disease. To investigate these aspects, researchers have employed recombinant DNA technology to produce the enzyme in a heterologous system, allowing for large-scale purification and characterization. The expression of the recombinant protein provides insights into substrate specificity, catalytic mechanisms, and potential inhibitors. By using techniques such as X-ray crystallography and molecular modeling, scientists aim to gain a detailed understanding of the enzyme's structure-function relationships, which can inform drug development and therapeutic interventions. This research not only sheds light on the molecular basis of metabolic disorders associated with phytanic acid but also contributes to the broader field of biochemistry and metabolic engineering.
